In addition, fluorescence expressed in transgenic petunia flowers was stable during long-term vegetative propagation. As expected, all transgenic plants exhibited brilliant green fluorescence easily visible to the naked eye without an emission filter. Here, to overcome this limitation, we generated transgenic petunia plants expressing eYGFPuv, a CpYGFP derivative exhibiting bright fluorescence under invisible ultraviolet (UV) light excitation, with a novel combination of transcriptional terminator plus translational enhancer. Although we previously generated transgenic torenia plants expressing green fluorescent protein from marine plankton (CpYGFP), in which bright fluorescence was easily visible at the whole plant level, the maximum excitation of this protein within the visible light spectrum required the use of a coloured emission filter to eliminate exciting light. The application of fluorescent proteins in ornamental plants has lagged behind despite the recent development of powerful genetic tools.
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